heat shock protein family a member 8 hspa8  (Santa Cruz Biotechnology)

Journal: Oncology Letters

Article Title: Comprehensive treatment for two patients with small cell neuroendocrine carcinoma of the liver: A case report and literature review

doi: 10.3892/ol.2026.15544

Figure Lengend Snippet: Case 2: Pathology of S5 hepatocellular carcinoma: High magnification reveals tumor cells that are polygonal in shape, with round nuclei, prominent nucleoli and cytoplasm exhibiting a finely granular appearance. (A) H&E (magnification, ×100; scale bar, 73 µm). Immunohistochemical results: (B) Arginase-1 (+), with arrows indicating cytoplasmic positivity in tumor cells; (C) hepatocyte paraffin 1 (+), with arrows indicating cytoplasmic granular positivity; (D) Ki-67 (~15%+ in active area), with arrows indicating nuclear positivity in proliferating tumor cells; (E) glutamine synthetase (+), with arrows indicating cytoplasmic positivity in a map-like distribution pattern; (F) glypican-3 (focally +), with arrows indicating focal cytoplasmic and membranous positivity; (G) heat shock protein 70 (focally +), with arrows indicating focal nuclear and cytoplasmic positivity; and (H) CD34 (showing diffuse capillary distribution), with arrows highlighting the characteristic sinusoidal endothelial staining pattern (magnification, ×40; scale bar, 221 µm).

Article Snippet: The following primary antibodies were used: Pan-cytokeratin (CKpan; cat. no. ZM-0069), vimentin (cat. no. ZM-0260), CgA (cat. no. ZA-0507), Syn (cat. no. ZA-0569), cluster of differentiation 56 (CD56; cat. no. ZM-0427), TTF-1 (cat. no. ZM-0270), arginase-1 (cat. no. ZA-0641), HepPar-1 (cat. no. ZM-0133), P53 (cat. no. ZM-0408), Ki-67 (cat. no. ZM-0166), CD34 (cat. no. ZM-0046), CK19 (cat. no. ZM-0074), alpha-fetoprotein (AFP; cat. no. ZM-0008), CK7 (cat. no. ZM-0071), CK20 (cat. no. ZM-0072), Villin (cat. no. ZM-0441), caudal type homeobox 2 (CDX2; cat. no. ZA-0520), MutL homolog 1 (MLH1; cat. no. ZA-0544), MutS homolog 2 (MSH2; cat. no. ZA-0542), MSH6 (cat. no. ZA-0546), postmeiotic segregation increased 2 (PMS2; cat. no. ZA-0548), glutamine synthetase (cat. no. ZA-0645), glypican-3 (cat. no. ZM-0446), heat shock protein 70 (HSP70; cat. no. ZA-0580; all ready-to-use; from OriGene Technologies, Inc.), and programmed cell death ligand 1 (PD-L1; cat. no. M3653; dilution 1:50; Dako; Agilent Technologies, Inc.).

Techniques: Immunohistochemical staining, Staining

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Infosomes as Inflammatory Mediators: Proteomic Profiling of Proteins Enriched in Inflammatory Extracellular Vesicles

doi: 10.1016/j.mcpro.2026.101511

Figure Lengend Snippet: Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.

Article Snippet: Primary antibodies used for immunoblotting included High Mobility Group Box 1 (HMGB1) (Abcam Limited, Cambridge, UK; Cat. No. ab18256), copine-1 (Novusbio; Centennial, Cat. No. NBP1-32194), TUBA4a (Cell Signaling Technology, Inc.; Cat. No. #3878), Heat Shock Protein Family A Member 8 (HSPA8) (Santa Cruz Biotechnology Cat. No. SC-7298), TSG101 (Abcam Limited; Cat. No. ab125011), GAPDH (Cell Signaling Technology, Inc.; Cat. No. #2118), NLRP3 (AdipoGen Life Sciences Cat. No. AG-20B-0014-C100), IL-1β (Cell Signaling Technology, Inc.; Cat. No. #12703 or #12426), cleaved IL-1β (Cell Signaling Technology, Inc.; Cat. No. #83186 or #52718), Caspase-1 (Cell Signaling Technology, Inc.; Cat. No. #2225 or AdipoGen Life Sciences; Cat. No. AG-20B-0042–100), ASC (Santa Cruz Biotechnology, Cat. No. SC-514414 or Cell Signaling Technology, Inc.; Cat. No. #67824), and β-Actin (Cell Signaling Technology, Inc.; Cat. No. #3700).

Techniques: Marker, Quantitative Proteomics, Expressing, Western Blot